| 產品名稱 |
Simian virus 40 |
| 商品貨號 |
B198525 |
| Designations |
pUCSV-VA45-54-1Ea [molecular clone of Simian virus 40, SV-40] |
| GenBank Number |
AF156107
|
| Species |
Simian virus 40, Simian virus 40 |
| Depositors |
JA Lednicky, JS Butel |
| Applications |
contains sequence |
| Vector |
Construct size (kb): 7.85699987411499 |
| Insert |
DNA: cDNA Insert lengths(kb): 5.171000003814697 Tissue: uninoculated grivet monkey cells Gene product: [SV-VA45-54-1Ea] |
| Insert Size (kb) |
5.171 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Insert contains restriction sites from 5'end: BamHI-0.771,7.086; EcoRI-2.686,5.171;KpnI-3.696,4.161; SalI-7.857; SfiI-7.857; XhoI-uncut. The plasmid size = 7857 bp [2686bp(pUC-19 vector) +5171bp(SV40-VA45-54-1insert)]. The variant of SV-40 strain VA-45-54 has an archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-1Ea DNA into ATCC CV-1 cells by the contributor. |
| Classification |
Polyomaviridae, Polyomavirus |
| References |
Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047
Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955
|
