| 產品名稱 |
SCS#47 |
| 商品貨號 |
B198733 |
| Designations |
SCS#47 |
| GenBank Number |
M93108
|
| Species |
Mus musculus, mouse |
| Depositors |
SC Sealfon |
| Applications |
produces protein gonadotropin-releasing hormone receptor |
| Vector |
Construct size (kb): 4.157999992370606 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.226999998092651 Tissue: pituitary alphaT3 cell line Gene product: gonadotropin-releasing hormone receptor [Gnrhr] Target Gene: gonadotropin-releasing hormone receptor |
| Insert Size (kb) |
1.227 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): KpnI--4.2; SacI--3.3, 0.9; HindIII--3.7, 0.62; PstI--3.7, 0.42, 0.28; EcoRI/XhoI--3.0, 1.3. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.22, 0.64; HindIII--0.5, 1.09; SacI--0.82. Linearizing the plasmid with XhoI and transcription from the T3 promoter gives the sense strand; linearizing with EcoRI and transcription from the T7 promoter gives the antisense strand. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Tsutsumi M, et al. Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor. Mol. Endocrinol. 6: 1163-1169, 1992. PubMed: 1324422
Stuart C Sealfon, personal communication
|
