| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 2.012, 5.306, 0.882, 0.413, 1.999, 0.976, 1.634, 1.517, 6.536, 0.538, 1.030, 1.374, 0.507, 2.434, 0.534, 6.513, 0.985, 0.730. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.42, 0.53, 0.73, 0.89, 1.0, 1.0, 1.4, 1.5, 1.7, 2.1, 2.5, 5.6, 6.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70098, 70403, 70487, 70886, 70899, 70923. |