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pcDNA3L-His-CAMYEL plasmid in <em>Escherichia coli </em>
pcDNA3L-His-CAMYEL plasmid in <em>Escherichia coli </em>
規(guī)格:
貨期:
編號(hào):B198924
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pcDNA3L-His-CAMYEL plasmid in Escherichia coli
商品貨號(hào) B198924
Designations pcDNA3L-His-CAMYEL plasmid in Escherichia coli
Depositors L Jiang
Biosafety Level 1
Host Escherichia coli Stbl2
Vector Information
Size (kb): 9.38
Vector: pcDNA3L-His-CAMYEL plasmid in E. coli (plasmid)
Type: expression
Promoters: Promoter for expression CMV
Marker(s): neoR, AmpR
Features: primer site: T7
transcription terminator: bGHpA
Comments A mammalian expression vector that includes CAMYEL (cAMP sensor using YFP-Epac-RLuc), a bioluminescence resonance energy transfer (BRET) sensor.

Vector features: CMV promoter: 232-819; 6xHis: 932-949; CAMYEL: 1025-4927; bGH pA: 4978-5202; SV40 promoter: 5681-6050; Neomycin resistance: 6086-6880; SV40 pA: 7054-7184 ; Amp resistance: 8385-9245

More information on this construct may be found at the AfCS website: www.signaling-gateway.org/data/plasmid/
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Jiang LI, et al. Use of cAMP BRET sensor to characterize a novel regulation of cAMP by sphingosine-1-phosphate/G13 pathway. J. Biol. Chem. 282: 10576-10584, 2007.

Shipped frozen
Shipping Information ?Frozen glycerol stock of E. coli containing the plasmid
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479