| 產品名稱 |
Canine adenovirus 1 |
| 商品貨號 |
B199048 |
| Classification |
Adenoviridae, Mastadenovirus, Canine adenovirus A |
| Deposited As |
Infectious canine hepatitis virus |
| Agent |
Canine adenovirus 1 |
| Strain |
Utrecht |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Lethal infection in dog, Netherlands, 1958 |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Images |
 |
| Effect on Host |
CPE, cell rounding and sloughing |
| Recommended Host |
MDCK (ATCC CCL-34) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-8 days |
| Name of Depositor |
JG Kapsenberg, Rijks Instituut Voor de Volksgezondheid, The Netherlands |
| Source |
Lethal infection in dog, Netherlands, 1958 |
| Year of Origin |
1958 |
| References |
Kapsenberg JG. Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Biol. Med. 101: 611-614, 1959. PubMed: 14404448
Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828
|