IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A cosmid vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References
Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634
