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pDP1125
pDP1125
規格:
貨期:
編號:B199101
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pDP1125
商品貨號 B199101
Designations pDP1125
GenBank Number

M26946

Species Homo sapiens, human
Depositors DC Page
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: male lymphoblastoid cell line WHT1659
Gene product: zinc finger protein, X-linked [ZFX]
Alleles: A1, A2, A1, A2
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0 (doublet); HindIII--6.2; BamHI--4.3, 2.1; SmaI--6.2; PstI--3.3, 1.1, 0.9, 0.88.
The GenBank accession M26946 contains the sequence from 1228 to 2443 of the sequence in the Nature paper.
pDP1125 is the 5' EcoRI fragment of cDNA2. It extends from nucleotide -426 through nucleotide +2592 of the published sequence. It includes the complete coding sequence with 5' and 3' flanking regions but does not include the Alu repeat.
The clone detects transcripts of 6.3 and 8.0 kb from ZFX. It encodes the protein of 804 amino acids.
The insert is oriented in the vector such that transcription from the T7 promoter gives the sense strand.
References

Schneider-Gadicke A, et al. ZFX has a gene structure similar to ZFY, the putative sex determinant, and excapes X inactivation. Cell 57: 1247-1258, 1989. PubMed: 2500252

Page DC, et al. Chromosomal localization of ZFX - a human gene that escapes X inactivation - and its murine homologs. Genomics 7: 37-46, 1990. PubMed: 1970799

Schneider-Gadicke A, et al. Putative transcription activator with alternative isoforms encoded by human ZFX gene. Nature 342: 708-711, 1989. PubMed: 2512506

David C Page, personal communication

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