A general purpose episomal Saccharomyces cerevisiae/Escherichia coli shuttle vector.
YEp6 is deleted of approximately 4 kb of 2 micron sequences and 200 bp of pBR322. It is stably maintained at high copy number in cir+ strains.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030
