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Murine leukemia virus
Murine leukemia virus
規格:
貨期:
編號:B199433
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Murine leukemia virus
商品貨號 B199433
Classification Retroviridae, Gammaretrovirus, Mammalian virus group
Deposited As Murine erythroleukemia virus
Agent Murine leukemia virus
Strain Rauscher
Applications
The R-SFFV clone can be rescued by superinfecting the host cells with Rauscher MuLV. Several weeks PI the cells will express R-SFFV and R-MuLV which can be injected into susceptible mice pretreated with phenyl hydrazin.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Biologically cloned NIH/3T3s infected with biologically cloned R-SFFV from Rauscher erythroleukemia cell line
Product Format frozen
Storage Conditions -70°C or colder
Comments
The R-SFFV clone can be rescued by superinfecting the host cells with Rauscher MuLV. Several weeks PI the cells will express R-SFFV and R-MuLV which can be injected into susceptible mice pretreated with phenyl hydrazin.
Effect on Host
No, in vitro effects: cytopathic effects in NIH/3T3 cells
Yes, in vivo effects: rescued R-SFFV causes proliferation of hematopoietic stem cells of mice in vivo or in vitro
Recommended Host
Mus musculus
mouse
Growth Conditions
Temperature: 37°C
Incubation: 3 days
Name of Depositor D Kabat
Special Collection ATCC
Source
Biologically cloned NIH/3T3s infected with biologically cloned R-SFFV from Rauscher erythroleukemia cell line
References

Machida CA, et al. Reduced leukemogenicity caused by mutations in the membrane glycoprotein gene of Rauscher spleen focus-forming virus. J. Virol. 49: 394-402, 1984. PubMed: 6319740

Ruta M, Kabat D. Plasma membrane glycoproteins encoded by cloned Rauscher and Friend spleen focus-forming viruses. J. Virol. 35: 844-853, 1980. PubMed: 7420541

Ruta M, et al. A murine leukemia virus mutant with a temperature-sensitive defect in membrane glycoprotein synthesis. Cell 16: 77-88, 1979. PubMed: 421271

Stephenson JR, Aaronson SA. Characterization of temperature-sensitive mutants of murine leukemia virus. Virology 54: 53-59, 1973. PubMed: 4123462

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