| 產(chǎn)品名稱 |
Measles virus |
| 商品貨號(hào) |
B199561 |
| Classification |
Paramyxoviridae, Morbillivirus |
| Agent |
Measles virus |
| Strain |
Edmonston |
| Applications |
A CE adapted Edmonston strain has been used for the production of vaccines for human use. Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Images |
 |
| Comments |
Measles, Rinderpest and Canine distemper viruses contain a common antigen. |
| Effect on Host |
CPE, syncytial rounding and sloughing |
| Recommended Host |
MRC-5 (ATCC® CCL-171™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-14 days |
| Name of Depositor |
JF Enders, Boston Children’s Hospital, Boston, Massachusetts |
| Special Collection |
NCRR Contract |
| Source |
Blood from patient in acute phase of typical measles, Massachusetts, 1954 |
| References |
Enders JF, Peebles TC. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc. Soc. Exp. Biol. Med. 86: 277-286, 1954. PubMed: 13177653
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