| 產(chǎn)品名稱 |
hMR(1-516) |
| 商品貨號(hào) |
B199746 |
| Designations |
hMR(1-516) |
| GenBank Number |
M80582
|
| Species |
Homo sapiens, human |
| Depositors |
MV Govindan |
| Vector |
Construct size (kb): 6.619999885559082 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: mLR
Genomic copy number: unique
Gene name: mineralocorticoid receptor (aldosterone receptor)
Contains complete coding sequence?: U
Chromosome: 4; Localization: 4 q31; 4, cR
Tissue: lymphocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.677
Cross references: DNA Seq. Acc.: M80582
Nucleotides 1-3677 of the insert correspond to
nucleotides 1-3677 of M80582. Insert lengths(kb): 3.677000045776367 Tissue: lymphocyte Gene product: mineralocorticoid receptor (aldosterone receptor) [MLR] |
| Insert Size (kb) |
3.677 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 3.4; EcoRI-- 4.0, 3.0; HindIII--4.3, 2.7; KpnI--4.4, 2.5; XbaI--4.5, 2.3. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--3.17; BglII--1.46; HindIII--1.0; KpnI--1.34; NcoI--0.67, 2.43; PstI--2.96; PvuII--2.54; SacI--2.63; XbaI--2.16. Contains regulatory elements and an open reading frame for aa 1-516 (nt 2124-3677), terminating at the EcoRI site. |
| References |
Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951
|
