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p79-2-23
p79-2-23
規(guī)格:
貨期:
編號:B199814
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p79-2-23
商品貨號 B199814
Designations p79-2-23
Species Homo sapiens, human
Depositors M Litt
Applications
Lathrop et al (HGM9) have used CEPH families to assign the following linkage group: cen- HP- CTRB- D16S20- pLC9- (APRT, D16S7)- qter.
Vector
Construct size (kb): 4.5
Insert
DNA: genomic
Insert lengths(kb): 1.450000047683716
Gene product: DNA Segment, single copy [D16S7]
Insert Size (kb) 1.450
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 1.5; PstI--3.2, 0.86; EcoRI--4.5; HindIII--4.5; SmaI--4.5.
Lathrop et al (HGM9) have used CEPH families to assign the following linkage group: cen- HP- CTRB- D16S20- pLC9- (APRT, D16S7)- qter.
Derived from cosmid CF33-79.
The number of alleles detected is a function of population size and gel resolution; in a study of >1000 unrelated individuals another lab claims to have detected over 100 alleles.
Use standard hybridization and washing conditions, with a final wash in 0.1X SSC/0.1% SDS at 65C.
References

Bufton L, et al. A highly polymorphic locus on chromosome 16q revealed by a probe from a chromosome-specific cosmid library. Hum. Genet. 74: 425-431, 1986. PubMed: 2878870

Chimera JA, et al. Population genetics of the highly polymorphic locus D16S7 and its use in paternity evaluation. Am. J. Hum. Genet. 45: 926-931, 1989. PubMed: 2574004

Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986

Michael Litt, personal communication

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