Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.2; EcoRI--3.1, 1.2; PstI--3.4, 0.87; SacI--2.8, 1.5; BglI--2.6, 1.7. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.3, 1.5; BglI--0.5; BglII--1.0, 1.05; EcoRI--1.05; NdeI--1.05; PstI--0.6; PvuII--0.15; SacI--1.45. Constructed from a SstI subfragment of pRatPPT-44. Contains a few nucleotides upstream of the initiation codon, the complete coding sequence, and approximately 500 bp of the 3' untranslated sequence. |
