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Vaccinia virus
Vaccinia virus
規格:
貨期:
編號:B200013
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Vaccinia virus
商品貨號 B200013
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain vP-22
Applications
produces protein thymidine kinase
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
A BglII-BamHI fragment encoding HSV-1 TK (minus the regulatory sequences) was cloned into one of the BamHI sites of an AvaI fragment from the vaccinia L-variant. This construct was then cloned into VTK-79L (ATCC VR-2056) by in vivo recombination.
Product Format frozen
Storage Conditions -70°C
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
A recombinant virus containing the HSV-1 Bgl/BamH1 TK-fragment in the vaccinia VTK-79L vector.
This clone shows a higher level of TK expression than variants vP-2, vP-4 (ATCC VR-2033), or vP-6 (ATCC VR-2029).
A BglII-BamHI fragment encoding HSV-1 TK (minus the regulatory sequences) was cloned into one of the BamHI sites of an AvaI fragment from the vaccinia L-variant. This construct was then cloned into VTK-79L (ATCC VR-2056) by in vivo recombination.
Effect on Host
Cytopathic effects in tissue culture
Name of Depositor E Paoletti
U.S. Patent
U.S. Patent Number
References

Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986

Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988

Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520

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