| 產品名稱 |
pHT10 |
| 商品貨號 |
B200055 |
| Designations |
pHT10 |
| Species |
Moloney murine sarcoma virus, Moloney murine sarcoma virus |
| Depositors |
GF Van de Woude |
| Vector |
Construct size (kb): 6.400000095367432 |
| Insert |
DNA: genomic Insert lengths(kb): 2.099999904632568 Gene product: Moloney murine sarcoma viral (v-mos) oncogene homolog [MOS] Alleles: B2, B1 |
| Insert Size (kb) |
2.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.0; EcoRI--6.4; PvuII--3.8, 2.6; BamHI--6.4. Very low transformation efficiency because lacks LTR. |
| Classification |
Retroviridae, Gammaretrovirus, Mammalian virus group |
| References |
Blair DG, et al. Biological activity of cloned Moloney sarcoma virus DNA: Terminally redundant sequences may enhance transformation efficiency. Proc. Natl. Acad. Sci. USA 77: 3504-3508, 1980. PubMed: 6251463
|
