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pSV2-cat
pSV2-cat
規格:
貨期:
編號:B200156
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pSV2-cat
商品貨號 B200156
Designations pSV2-cat
Depositors BH Howard
Other IDs

Nucleotide (GenBank) : VB0065 pSV2-Cat cloning vector.

Biosafety Level 1
Vector Information
Size (kb): 5.1
Vector: pSV2-cat (plasmid)
Promoters: Promoter SV40
Construction: pSV2-betaG, Tn9
Marker(s):ampR,cmlS
Construct size (kb): 5.1
Features: marker(s): ampR, cmlS
promoter: SV40
replicon: pMB1
Applications
expression vector
shuttle vector
Comments
Restriction digests of the vector give the following sizes (kb): HindIII--5.0; BamHI--5.0; BamHI/HindIII--3.5, 1.6; AccI--5.0.
Expresses chloramphenicol acetyltransferase in eukaryotic cell lines under the control of the simian virus 40 early promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240

Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651

Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479