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lambdaZd35
lambdaZd35
規格:
貨期:
編號:B200240
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 lambdaZd35
商品貨號 B200240
Designations lambdaZd35
Depositors AJ Murphy
Biosafety Level 1
Vector Information
Size (kb): 43.7000007629394500
Vector: lambdaZd35 (phage, lambda - replacement)
Promoters: Promoter Mo-MLV
Construction: Charon 30; pZIP-neoSV(x)1
Marker(s):G418R,ampR,kanR,neoR
Construct size (kb): 43.70000076293945
Features: marker(s): ampR, kanR, neoR, G418R
promoter: Mo-MLV, polyoma
replicon: lambda
terminator: 3' LTR
Applications
expression vector
lambda vector allowing conversion to plasmid
shuttle vector
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25.
A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817.
The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in Escherichia coli RRI(pRK248), ATCC 47036.
Allows expression of cloned cDNA in mammalian cells; can be used for transduction. Allows plasmid replication in mouse (WOP) and monkey (COS) cells.
Cloned cDNA expressed from LTR promoter; neo expressed from polyoma promoter; no splicing site; ori of SV40 and pBR322.
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

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