| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--18.0, 7.6, 7.2, 7.0, 4.2, 2.8, 1.6, 0.54; EcoRI--21.0, 11.0, 4.8, 3.6, 3.5, 2.3, 1.5; HindIII--21.0, 8.2, 6.6 (doublet), 2.5, 1.9, 1.3, 1.1; XbaI--25.0, 17.0, 5.8; KpnI--18.0, 9.4, 6.6, 5.0, 4.0, 2.6, 2.5, 1.8; SstI--36.0, 8.6, 4.6, 1.3. This insert contains 5' flanking sequences and exon 1. There is an EcoRI and a BamHI (exon 1) site in the insert. |