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Construct size (kb): 45.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPP3CB
Other gene symbols: CALNA2
Genomic copy number: unique
Gene name: protein phosphatase 3 (formerly 2B), catalytic subunit,
beta isoform (calcineurin A beta)
Contains complete coding sequence?: U
Chromosome: 10; Localization: 10; 10, 448.339999 cR
Tissue: brainstem
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M29551

Insert lengths(kb): 1.799999952316284

Tissue: brainstem

Gene product: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)(protein phosphatase 3, catalytic subunit, beta isoform) [CALNA2]

Restriction digests of the clone give the following sizes (kb): EcoRI--24.2, 19.5, 1.8; ClaI--12.5, 6.0 (doublet), 4.0 (doublet), 3.3, 2.6, 1.85, 1.6, 1.1 (doublet), 0.95, 0.6, 0.35; HindIII-->23.0, 9.4, 7.2, 3.8, 0.56;

PstI--12.5 (doublet), 4.7, 3.1, 2.85, 2.5, 2.0, 1.7, 1.15, 1.1, 0.8, 0.55, 0.48, 0.37 +smaller; PvuII-->23.0, 4.4, 4.2, 3.6, 2.7, 1.75, 1.2, 0.95, 0.6, 0.52.

The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.67; HindIII--0.37, 0.38; NcoI--0.11; PstI--1.01, 1.24; PvuII--1.00; SacI--0.68.

Corresponds to nt 311-2180 of the GenBank record, and includes the 3' portion of the coding region (nt 117-1691).

This was derived from the more prevalent type II isoform, and lacks 54 bp and encodes a different carboxy terminus relative to the type I.