Total size of construct (kb): 16.524
Marker: ampR
Vector: pAT153
Vector type: cloning vector
Insert site: BamHI
Vector size (kb): 3.658
Suggested primers for sequencing that flank BamHI site:
Primer1 (forward): 5’-CCTGCTCGCTTCGCTACTTG-3’
Primer2 (reverse): 5’-AGGCGCCAGCAACCGCACCT-3’
Insert
Insert size (kb): 12.866
Insert ends: BamHI
DNA: partial genomic DNA
Source: Molluscum contagiosum virus (MCV)
Genbank accession: U60315, partial
Nucleotides 1-12866 of the insert correspond to nucleotides 30222-43088 of Genbank U60315
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed 200 ng purified plasmid DNA
Comments
This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The 12.9 kb insert includes nucleotides 30222-43088 of Genbank sequence U60315 for the complete genome of the virus.
References
Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497
Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425
Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026
