| 產品名稱 |
hPAR2 cDNA |
| 商品貨號 |
B200476 |
| Designations |
hPAR2 cDNA |
| GenBank Number |
Z49993
Z49994
|
| Species |
Homo sapiens, human |
| Depositors |
JB Sundelin |
| Applications |
in another host, produces protein G-protein coupled receptor 11 proteinase-activated receptor 2 |
| Vector |
Construct size (kb): 5.360000133514404 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSG5
Intact vector size: 4.076
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI
Polylinker sites: EcoRI BamHI BglII
Construction: pKCR2, Bluescribe M13+
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
restriction site: SalI
promoter for expression: SV40 early, ->
other: rabbit beta-globin intron II, ->
promoter for in vitro transcription: T7, ->
MCS: EcoRI...BglII, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1, <-
marker(s): ampR, <-
replicon: M13, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPR11
Other gene symbols: PAR2*
Gene name: G-protein coupled receptor 11
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q13
Tissue: stomach
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 1.283
Cross references: DNA Seq. Acc.: Z49993
Nucleotides 1-91 of the insert correspond to
nucleotides 195-285 of Z49993.
DNA Seq. Acc.: Z49994
Nucleotides 92-1283 of the insert correspond to
nucleotides 97-1288 of Z49994. Insert lengths(kb): 1.282999992370606 Tissue: stomach Gene product: G-protein coupled receptor 11(proteinase-activated receptor 2) [GPR11] Target Gene: G-protein coupled receptor 11 |
| Insert Size (kb) |
1.283 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 1.3; EcoRI--5.4; BglI--2.1, 1.6, 1.1, 0.45. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.35; NcoI--0.55, 0.83; PstI--0.07; PvuII--1.02. Insert was amplified by PCR of total RNA from human stomach, followed by restriction digestion with BamHI. |
| References |
Nystedt S, et al. Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor2. Eur. J. Biochem. 232: 84-89, 1995. PubMed: 7556175
|
