黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

Size (kb): 6.43

Vector: pHC79 (cosmid)

Construction: pBR322, Charon 4A

Marker(s):ampR,tetR

Construct size (kb): 6.43

Features: marker(s): ampR, tetR
replicon: pMB1
terminator: none

vector for constructing cDNA libraries

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

General purpose cosmid vector derived from pBR322.

pBR322 was partially digested with Sau3A and ligated with an 0.65 BglII lambda fragment, generating a BglII site (pBR322 positions 1459-1666 deleted). A 1.65 kb BglII fragment from Charon 4A was ligated into the BglII site.

Temperature: 30.0°C