| 產品名稱 |
pSCH038 |
| 商品貨號 |
B200621 |
| Designations |
pSCH038 |
| GenBank Number |
V00348
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
KJ Shaw |
| Applications |
The 742bp EcoRI fragment is recommended as a hybridization probe for the 16S rRNA gene. |
| Vector |
Construct size (kb): 4.639999866485596 |
| Insert |
DNA: Synthetic Insert lengths(kb): 0.7369999885559082 Gene product: 16S rRNA [16SrRNA*] |
| Insert Size (kb) |
0.737 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 0.9; HindIII--5.3; BamHI--5.3. Two rounds of gel purification of the restriction digest generated probe is necessary. Failure to do so often results in high backgrounds (due to flanking regions in the plasmid insert) and false positives with clinical E. coli strains. The 742bp EcoRI fragment is recommended as a hybridization probe for the 16S rRNA gene. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996
|
