| Insert |
DNA: cDNA Insert lengths(kb): 0.8399999737739563 Tissue: bladder carcinoma T24 cell line Gene product: GRO1 oncogene (melanoma growth stimulating activity, alpha)(growth regulated (alpha), growth regulated (group I)) [GRO] Alleles: A1, A2, A1, A1, A2, A2, A1, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII--3.5, 0.2; BamHI--3.1, 0.6; PstI--3.4, 0.3; PvuII--3.1, 0.6. Contains the EcoRI fragment corresponding to nt 205 to nt 1050 of the published sequence. The insert includes the following restriction sites in nt from the 5' end: HindIII--96; PvuII--257, PstI--262; BamHI--568. The insert is inserted such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand. Enzyme(s) not detecting polymorphism: AluI, AlwNI, ApaI, ApaLI, AseI, AvaI, BamHI, BanII, BclI, BglI, BglII, BspHI, BstUI, DraI, Eco0109, EcoRI, HaeIII, HincII, HindIII, HphI, KpnI, MspI, NraI, PstI, PvuII, RsaI, SacI, Sau3AI, SmaI, StuI, TaqI, XbaI. |
| References |
Beck JS, et al. A ScaI RFLP demonstrated for the GRO gene on chromosome 4. Nucleic Acids Res. 17: 8895, 1989. PubMed: 2573881
Haskill S, et al. Identification of three related human GRO genes encoding cytokine functions. Proc. Natl. Acad. Sci. USA 87: 7732-7736, 1990. PubMed: 2217207
Anisowicz A, et al. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells. Proc. Natl. Acad. Sci. USA 84: 7188-7192, 1987. PubMed: 2890161
Anthony Anisowicz, personal communication
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