| 產品名稱 |
pAS1-p53 |
| 商品貨號 |
B201088 |
| Designations |
pAS1-p53 |
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Applications |
in another host, produces protein tumor protein p53 (Li-Fraumeni syndrome) tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA binding domain, ->
epitope tag: HA (hemagglutinin), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TP53
Genomic copy number: unique
Gene name: tumor protein p53 (Li-Fraumeni syndrome)
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p13.1
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb):
Cross references: Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) [TP53] Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2 Target Gene: tumor protein p53 (Li-Fraumeni syndrome) |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): XhoI--6.0, 1.25, 0.95; BamHI/HindIII--5.0, 1.45, 0.95, 0.75; EcoRI-- 8.2; BamHI--8.2; PstI--7.2, 0.95. Used as a negative control in the 2-hybrid cloning system. |
| References |
Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751
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