Insert contains PstI, HindIII, AvaI and SalI sites. The insert contains the following sites separated by (bp)(approx): EcoRI- 175- PstI, HindIII- 520- PstI- 175- AvaI- 300- PstI- 175- SalI- 175- SalI- 910- SalI- 130- EcoRI. This is a clone of the nifK gene, including the wild type alleles for nif-6 and nif-38. |