| 產品名稱 |
EC-AM1 [AM1, ECAM1] |
| 商品貨號 |
B201406 |
| Designations |
EC-AM1 [AM1, ECAM1] |
| Species |
Hepatitis B virus, Hepatitis B virus |
| Depositors |
Scripps Clinic and Research Foundation, AM Moriarity, Scripps Clinic and Research Foundation |
| Applications |
This material is cited in a U.S. and/or other patent Application and may not be used to infringe the patent claims. |
| Vector |
Construct size (kb): 6.210000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: hepatitis B virus
Gene symbol: cAg
Gene name: core antigen
Contains complete coding sequence?: U
Genome: hepatitis B virus
Gene symbol: xAg
Gene name: X antigen; X gene product
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 1.85
Cross references: Insert lengths(kb): 1.850000023841858 Gene product: X antigen; X gene product [cAg] |
| Insert Size (kb) |
1.850 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.8; HindIII--6.2; EcoRI--6.2. Contains gene for HBV X antigen. This material is cited in a U.S. and/or other patent Application and may not be used to infringe the patent claims. Molecular clone of hepatitis B BamHI fragment EC-AM1 (ATCC 39629) and SVAM191 (ATCC 40103, 39631) contain the same BamHI fragment of hepatitis B virus. The insert extends from the BamHI site at 1400 of the hepatitis B virus map to the BamHI site at 28. |
| Classification |
Hepadnaviridae, Orthohepadnavirus |
| References |
Moriarty AM. SV40 expression vector containing HBxAg as an expression marker. US Patent 4,942,125 dated Jul 17 1990
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
