| 產品名稱 |
pALCA1S |
| 商品貨號 |
B201614 |
| Designations |
pALCA1S |
| Depositors |
Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli JM83; K-12 strain JM83 |
| Vector Information |
Size (kb): 4.5000000000000000 Vector: pALCA1S (plasmid) Promoters: Promoter alcA Construction: pDG6 (ATCCC 53169) Marker(s):ampR,argB Construct size (kb): 4.5 Features: marker(s): ampR
marker(s): argB
promoter: alcA
replicon: pMB1
MCS: XbaI...EcoRI
signal peptide coding region: synthetic |
| Applications |
expression vector vector with sequences for protein export |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; BamHI--4.5; HindIII--4.5. Constructed from pDG6 (ATCC 53169) by removing the endoglucanase coding region and the initiator codon of the alcA, retaining the TATA box and the transcription start site. A synthetic signal peptide coding region was inserted. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
