| 產(chǎn)品名稱 |
pSREBP-2 |
| 商品貨號 |
B201787 |
| Designations |
pSREBP-2 |
| GenBank Number |
U02031
U12330
|
| Species |
Homo sapiens, human |
| Depositors |
JL Goldstein |
| Vector |
Construct size (kb): 8.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pEXlox(+)
Intact vector size: 4.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SREBP2
Gene name: sterol regulatory element binding transcription factor 2
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 4.249
Cross references: DNA Seq. Acc.: U02031
Nucleotides 1-4249 of the insert correspond to
nucleotides 1-4249 of U02031. Insert lengths(kb): 4.249000072479248 Gene product: sterol regulatory element binding transcription factor 2(sterol regulatory element-binding protein 2) [SREBP2] |
| Insert Size (kb) |
4.249 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.2, 3.0; EcoRV--8.2; SalI--4.25, 3.95; EcoRI--3.95, 2.6, 1.65; EcoRI/SalI--3.95, 2.6, 1.65. The insert contains the following restriction sites (bp from the 5' end): EcoRI--2614; EcoRV--1142; BamHI--2170. The fragment excised by a SalI digest contains 6 bp of adaptor sequence. |
| References |
Hua X, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA : 11603-11607, 1993. PubMed: 7903453
Hua X, et al. Structure of the human gene encoding sterol regulatory element binding protein-1 (SREBF1) and localization of SREBF1 and SREBF2 to chromsosomes 17p11.2 and 22q13. Genomics 25: 667-673, 1995. PubMed: 7759101
Joseph L Goldstein, personal communication
|
