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pHG276
pHG276
規格:
貨期:
編號:B201911
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pHG276
商品貨號 B201911
Designations pHG276
Depositors GD Stewart
Biosafety Level 1
Vector Information
Size (kb): 4.3200001716613770
Vector: pHG276 (plasmid)
Promoters: Promoter lambda PR
Construction: pHG165, lambdaPR
Marker(s):ampR
Construct size (kb): 4.320000171661377
Features: insert detection: lacZ'
marker(s): ampR
promoter: lambda PR
replicon: pMB1
repressor gene: cI857
Applications
expression vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HpaI--3.0, 1.3.
Plasmid expression vector with lambda PR promoter, plasmid copy number controlled by rop(rom), and cI857 for temperature regulation of lac and insert expression.
A 1.7 kb BamHI/HpaI fragment containing the cI gene and lambda PR was cloned into BamHI/HpaI partially digested pHG165 (ATCC 37618).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Stewart GS, et al. pHG276: a multiple cloning site pBR322 copy number vector expressing a functional lacalpha peptide from the bacteriophage lambda PR promoter. Plasmid 15: 182-190, 1986. PubMed: 3012612

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