| 產品名稱 |
pN29III |
| 商品貨號 |
B202098 |
| Designations |
pN29III |
| GenBank Number |
X56974
|
| Species |
Mus musculus, mouse |
| Depositors |
I Oberbaumer |
| Applications |
standard for analysis of gene expression RNA, ribosomal, 18S |
| Vector |
Construct size (kb): 3.450000047683716 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.7519999742507935 Tissue: teratocarcinoma PYS-2/12 cell line Gene product: RNA, ribosomal, 18S [Rn18s] Target Gene: RNA, ribosomal, 18S |
| Insert Size (kb) |
0.752 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/SphI--2.7, 0.75; XbaI--3.3, 0.13; SmaI--2.75, 0.35, 0.3; SacI--3.4, 0.45. The insert contains the following restriction sites (approximate kb from the 5' end): XbaI--0.32, 0.45; SmaI--0.36, 0.66; SacI--0.40. Extends from nt 1032 to 1784 of the reported sequence. Includes an additional nt at position 1476. Subcloned from pN29. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975
Oberbaumer I. Retroposons do jump: a B2 element recently integrated in an 18S rDNA gene. Nucleic Acids Res. 20: 671-677, 1992. PubMed: 1311830
Ilse Oberbaumer, personal communication
|
