| 產品名稱 |
p4 |
| 商品貨號 |
B202153 |
| Designations |
p4 |
| GenBank Number |
L10918
|
| Species |
Homo sapiens, human |
| Depositors |
PM Murphy |
| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CMKBR1
Gene name: chemokine (C-C) receptor 1
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p21
Tissue: HL-60 cell line
Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 2.156
Cross references: DNA Seq. Acc.: L10918
Nucleotides 1-2156 of the insert correspond to
nucleotides 1-2156 of L10918. Insert lengths(kb): 2.155999898910523 Tissue: HL-60 cell line promyelocytic leukemia Disease: promyelocytic leukemia Gene product: chemokine (C-C) receptor 1( chemokine (C-C) receptor 1, RANTES receptor) [CMKBR1] |
| Insert Size (kb) |
2.156 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0, 2.2; EcoRI--4.6, 0.6; XhoI--5.2; EcoRI/XhoI--3.0, 1.6, 0.6; PstI--4.6, 0.6. The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.45; EcoRI--0.59; PstI--0.61. |
| References |
Gao JL, et al. Structure and functional expression of the human macrophage inflammatory protein 1alpha/RANTES receptor. J. Exp. Med. 177: 1421-1427, 1993. PubMed: 7683036
|
