Restriction digests of the clone give the following sizes (kb): SacI/XbaI--2.7, 0.9; EcoRI--3.1, 0.6; BamHI--3.6. The insert contains the following restriction sites (approximate kb from the 5' end): NcoI--0.48, 0.66; EcoRI--0.6; KpnI--0.65; XmnI--0.72; BamHI--0.91. The plasmid is not stable in E. coli MC1061. Corresponds to nt 2593-3510 of the virus, including the complete coding sequence for the coat protein. Transcription from the T7 promoter gives the sense (+) strand. |
