| 產品名稱 |
pAW739 |
| 商品貨號 |
B202297 |
| Designations |
pAW739 |
| Species |
Homo sapiens, human |
| Depositors |
Cetus Corp., A Belt, Cetus Corp. |
| Vector |
Construct size (kb): 7.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pFC54.t
Intact vector size: 5.610
Type of vector: plasmid
Vector end: HindIII
Vector end: BamHI
Cloning sites: BamHI HindIII
Polylinker sites:
Construction: pFC54, pHCW801
Features (with orientation and position when available): |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: TNFA
Genomic copy number: unique
Gene name: tumor necrosis factor, alpha (cachectin)
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 p21.3
Type of DNA: cDNA
Insert end: HindIII
Insert end: BamHI
Insert size (kb): 1.3
Cross references: Insert lengths(kb): 1.299999952316284 Gene product: tumor necrosis factor, alpha (cachectin) [TNFA] |
| Insert Size (kb) |
1.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--5.8, 0.75, 0.6; HindIII--6.6, 0.6. A 0.6 kb HindIII fragment contains the coding sequence plus some 3' untranslated sequence. This should not be grown at temperatures exceeding 30C. This was constructed from pAW711 (ATCC 39918) by deleting nucleotides encoding 6 amino terminal amino acids starting with Val of the processed TNF. This product shows maximal biological activity. |
| References |
Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,063 dated Jun 30 1987
Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,064 dated Jun 30 1987
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
