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pMF-alpha8 [pMFalpha8]

pMF-alpha8 [pMFalpha8]

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產(chǎn)品名稱	pMF-alpha8 [pMFalpha8]
商品貨號(hào)	B202315
Designations	pMF-alpha8 [pMFalpha8]
Depositors	A Miyajima
Biosafety Level	1
Vector Information	Size (kb): 7.0000000000000000 Vector: pMF-alpha8 (plasmid) Promoters: Promoter alpha factor 1 Construction: pTRP584, pMFalpha5 Marker(s):TRP1,ampR Construct size (kb): 7.0 Features: marker(s): ampR, TRP1 promoter: alpha factor 1 replicon: pMB1, 2 micron, ARS1 terminator: TRP5
Applications	YX-type (expression) shuttle vector expression vector shuttle vector vector with sequences for protein export
Comments	Shuttle expression vector permitting processing and secretion of eukaryotic proteins. Cloning sites are immediately downstream of the first processing site for the alpha factor leader. Constructed by inserting a modified alpha promoter leader sequence into pTRP584, replacing the StuI site in the TRP1-ARS1 fragment with a PvuII site, so that the StuI site in the alpha factor processing region is unique. The order of the major features in this plasmid is: 2 micron ori - alpha factor leader - StuI - XhoI - TRP5 - EcoRI - pMB1 ori - ampR - EcoRI - ARS1 - PvuII - TRP1 - EcoRI.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Miyajima A, et al. Secretion of mature mouse interleukin-2 by Saccharomyces cerevisiae: use of a general secretion vector containing promoter and leader sequences of the mating pheromone alpha-factor. Gene 37: 155-161, 1985. PubMed: 3902570
Shipped	freeze-dried

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