IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Broad host range cosmid vector which can be selected and replicate in Escherichia coli and Vibrio parahaemolyticus.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References
Datta AR, et al. Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus. J. Bacteriol. 160: 808-811, 1984. PubMed: 6094492
