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Leishmania tropica (Wright) Luhe
Leishmania tropica (Wright) Luhe
規格:
貨期:
編號:B202634
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Leishmania tropica (Wright) Luhe
商品貨號 B202634
Deposited As Leishmania adleri
Strain Designations WHO Israel LRC 146
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
lizard, Latastia longicauda revoili, Kenya
Product Format frozen
Type Strain no
Comments
Taxonomy (ATCC lab.). Promastigotes.
In vitro phagocytosis by lizard leukocytes
Taxonomic status
Variation in kinetoplast minicircle and maxicircle DNA sequences
Cyclopropane fatty acid
Medium ATCC® Medium 811: Leishmania medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor J Janovy
Chain of Custody
ATCC <<--J Janovy<<--WHO LRC Jerusalem <<--- . . . <<--- Heisch?
References

Dollahon NR, Janovy J Jr.. Leishmania adleri: in vitro phagocytosis by lizard leukocytes and peritoneal exudate cells. Exp. Parasitol. 34: 56-61, 1973. PubMed: 4722485

Dollahon NR, Janovy J Jr.. Experimental infection of New World lizards with Old World lizard Leishmania species. Exp. Parasitol. 36: 253-260, 1974. PubMed: 4413377

Simpson L. The status of Leishmania tarentolae/Trypanosoma platydactyli. Parasitol. Today 4: 115-118, 1988.

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003

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