Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2; BamHI/EcoRI--4.5, 0.7.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Chedid M, Mizel SB. Involvement of cyclic AMP-dependent protein kinases in the signal transduction pathway for interleukin-1. Mol. Cell. Biol. 10: 3824-3827, 1990. PubMed: 2192264