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pB12BSM
pB12BSM
規格:
貨期:
編號:B202783
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pB12BSM
商品貨號 B202783
Designations pB12BSM
Species Beet necrotic yellow vein virus
Depositors KE Richards
Vector
Construct size (kb): 5.019999980926514
Insert
DNA: cDNA
Insert lengths(kb): 1.820000052452087
Gene product: genome, partial
Insert Size (kb) 1.820
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PvuII--3.0, 1.7.
To synthesize antisense RNA in vitro, cut with EcoRI and transcribe from vector T3 promoter.
The insert is nt 4740-6560 of RNA-1 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious.
Classification Furovirus
References

Bouzoubaa S, et al. Nucleotide sequence of beet necrotic yellow vein virus RNA-1. J. Gen. Virol. 68: 615-626, 1987.

Bouzoubaa S, et al. Shortened forms of beet necrotic yellow vein virus RNA-3 and RNA-4: internal deletions and a subgenomic RNA. J. Gen. Virol. 72: 259-266, 1991. PubMed: 1993870

Ken E Richards, personal communication

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