Total size of construct (kb): 7.97
Marker: ampR
Vector: pAT153
Vector type: cloning vector
Insert site: HindIII
Vector size (kb): 3.658
Markers: ampR
Suggested primers for sequencing that flank HindIII site:
Primer 1 (forward):
5’-AACCTATAAAAATAGGCGTATCACG-3’
Primer 2 (reverse):
5’-CGCATTGTTAGATTTCATACACGG-3’
Insert
Insert size (kb): 4.165 + 0.146 (4.311 kb total)
Insert ends: HindIII
DNA: partial genomic DNA
Source: Molluscum contagiosum virus (MCV)
Genbank accession: U60315, partial
Nucleotides 1-4165 and 4166-4311 of the insert correspond to nucleotides 162560-166725 and 187252 – 187398 respectively, of Genbank U60315
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
200 ng DNA
Comments
This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The 4.2 and 0.146 kb insert includes nucleotides 162560-166725 and 187252-187398 of Genbank sequence U60315 for the complete genome of the virus.
References
Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497
Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026
Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425
