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pRML1
pRML1
規(guī)格:
貨期:
編號(hào):B203335
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRML1
商品貨號(hào) B203335
Designations pRML1
Depositors F Spencer
Biosafety Level 1
Host Escherichia coli DH5α
Vector Information
Size (kb): 9.6999998092651370
Vector: pRML1 (plasmid)
Promoters: Promoter for in vitro transcription T3
Construction: This plasmid was derived from pCGS966, and includes a TRP1 selectable marker, yeast origin of replication ARS1.
Marker(s): ampR
Cloning sites: BamHI NdeI SpeI ClaI SalI NotI EcoRI

Selectable marker cassette: TRP1, ←
Replicon: ARS1, ←
Insert detection: GAL, ←
Centromere: CEN4, ←
Promoter for in vitro transcription: T3, ←
Replicon: ori, ←
Marker: ampR, ←
Coding sequence: TK, ←
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.7; EcoRI--9.7, BamHI/EcoRI--8.9, 0.8.
The plasmid was derived from pCGS966, and includes a TRP1, ARS1, features for YAC amplification, a distal polylinker to support plasmid rescue of insert ends, T3 promoter, and a cloning polylinker for library production. Designed for use with pRML2.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Smith DR, et al. Amplification of large artificial chromosomes. Proc. Natl. Acad. Sci. USA 87: 8242-8246, 1990. PubMed: 2236036

Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993.

Smith DR, et al. Copy number amplification of yeast artifical chromosomes. Methods Enzymol. 216: 603-614, 1992. PubMed: 1336106

Zhong TP, et al. Zebrafish genomic library in yeast artifical chromosomes. Genomics 48: 136-138, 1998. PubMed: AMBIGUOUS 95

Haldi ML, et al. A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome. Mamm. Genome 7: 767-769, 1996. PubMed: 8854865

Shipped freeze-dried
Shipping Information in E. coli containing the plasmid
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479