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Size (kb): 3.0269999504089360

Vector: pDK101 (phagemid)

Promoters: Promoter for in vitro transcription T7

Construction: pGEM-5Zf(+), synthetic adaptor

Marker(s):ampR

Construct size (kb): 3.026999950408936

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: ApaI...NsiI

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector useful for cloning PCR products

Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0.

The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014.

A high copy number vector useful for direct cloning of double stranded amplification products.

Digestion of the vector with XcmI produces a linear molecule with 3' unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products.

Temperature: 37.0°C