| Comments |
The insert includes the following restriction sites (in bp from the 5' end): XhoI (671), PstI (711), and BamHI (1718). Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.0; BamHI--3.2, 2.0; PstI--3.2, 2.0; XhoI--5.2; SalI--5.2. Contains the complete coding sequence of TGFB1, including 779 bp 5' untranslated sequence, 179 bp 3' untranslated sequence, and a poly(A) tract of 37 bp. Based on this, it appears to correspond roughly to nt 55-2200 of GenBank X02812. This probe detects a mRNA of 2.5 kb in peripheral blood mononuclear cells and LAK precursors. May cross hybridize with 28S ribosomal subunit. Enzyme(s) not detecting polymorphism: NruI, NsiI, PaeR7I, PstI, PvuII, RsaI, RsrII, SacI, SalI, Sau3AI, Sau96I, ScaI, SmaI, SpeI, SspI, StuI, StyI, TaqI, XbaI, XboI, XmnI (with DNA from 7 unrelated Caucasians). Enzyme(s) not detecting polymorphism: AvaII, BamHI, BanII, BclI, BglI, BglII, BstEI, BstNI, ClaI, Cfr13I, DraI, Eco0109, EcoRI, EcoRV, FokI, HaeII, HaeIII, HincII, HindIII, HinfI, HinpI, HpaI, HphI, KpnI, MboI, MboII, MspI, NciI, NdeI, NheI. |
