Restriction digests of the clone give the following sizes (kb): EcoRI--14.0; HindIII--14.0.
Broad host range expression vector controlled by the tac promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Bagdasarian MM, et al. Activity of the hybrid trp-lac(tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene 26: 273-282, 1983. PubMed: 6323265
