| 產品名稱 |
pKKPAH.T87S |
| 商品貨號 |
B203600 |
| Designations |
pKKPAH.T87S |
| GenBank Number |
M12337
|
| Species |
Rattus norvegicus, rat |
| Depositors |
JP Caradonna |
| Applications |
in suitable host, produces protein phenylalanine hydroxylase |
| Vector |
Construct size (kb): 4.796999931335449 |
| Insert |
DNA: Synthetic Insert lengths(kb): 1.368000030517578 Gene product: phenylalanine hydroxylase [PAH] Target Gene: phenylalanine hydroxylase |
| Insert Size (kb) |
1.368 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, SalI--4.8; EcoRI/SalI--3.5, 1.4. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.17; NcoI--0.51; NdeI--1.02; PstI--0.70, 1.34; PvuII--0.01, 0.68; StuI--0.58; XhoI--0.75. Constructed by amplifying a product from the clone pRPH3. This contains a missense mutation (ACG->AGC) with respect to the GenBank sequence (M12337), resulting in serine at codon 87 rather than threonine. |
| References |
Kappock TJ, et al. Spectroscopic and kinetic properties of unphosphorylated rat hepatic phenylalanine hydroxylase expressed in Escherichia coli. J. Biol. Chem. 270: 30532-30544, 1995. PubMed: 8530485
Dahl HH, Mercer JF. Isolation and sequence of a cDNA clone which contains the complete coding region of rat phenylalanine hydroxylase. Structural homology with tyrosine hydroxylase, glucocorticoid regulation, and use of alternate polyadenylation sites. J. Biol. Chem. 261: 4148-4153, 1986. PubMed: 2869038
|
