| 產品名稱 |
HHCPR19 |
| 商品貨號 |
B203603 |
| Designations |
HHCPR19 |
| GenBank Number |
M78213
|
| Species |
Homo sapiens, human |
| Applications |
The predicted product size is (bp): 98. The observed product size(s) is(are) (bp): 99 (human). |
| Vector |
Construct size (kb): 5.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PHB
Gene name: prohibitin
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q21
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.85
Cross references: DNA Seq. Acc.: M78213; EST: EST01806; Primer: M13 Forward;
THC id: THC123454
DNA Seq. Acc.: S85655
Nucleotides 1-369 of the insert correspond to
nucleotides 124-492 of S85655 with 99% identity. Insert lengths(kb): 2.849999904632568 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: prohibitin [PHB] |
| Insert Size (kb) |
2.850 |
| Related Products |
plasmid DNA:ATCC 73227 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.5, 0.9 (doublet). The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. Based on the alignment with S85655 (1043 nt, CDS 51-869) the sequence generated from the M13 forward (M13-20) primer begins within the coding region. EST01806 can be detected in genomic DNA using the following oligonucleotides (5'-3'): ATAATGTGGATGCTGGGC and CCACGGGATGAGAAAATG. The predicted product size is (bp): 98. The observed product size(s) is(are) (bp): 99 (human). Related product(s): oligonucleotides, ATCC 73227. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994
|
