| Vector |
Construct size (kb): 7.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA3.1(-)
Intact vector size: 5.400
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: NheI ApaI XbaI XhoI NotI EcoRV EcoRI BamHI Asp718I KpnI
HindIII AflII
Polylinker sites: NheI PmeI ApaI XbaI XhoI NotI BstXI EcoRV EcoRI BstXI BamHI
Asp718I KpnI HindIII AflII PmeI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for expression: CMV, ->, 209-863
promoter for in vitro transcription: T7, ->, 863-882
MCS: NheI...HindIII, ->, 895-110
primer site: pcDNA3.1 reverse primer, <-, 1022-1039
terminator: bGH polyadenylation, ->, 1021-1235
replicon: f1, ->, 1298-1711
replicon: SV40, ->, 1776-2055
promoter: SV40, ->, 2056-2101
marker(s): G418R, ->, 2137-2931
terminator: SV40 polyadenylation, ->, 2986-3358
replicon: pMB1, 3618-4291
marker(s): ampR, <-, 4436-5297
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CMV-His-ACS
Gene name: acetyl-CoA synthetase
Contains complete coding sequence?: Y
Tissue: human
Type of DNA: cDNA
Insert end: BamHI
Insert end: EcoRI
Insert size (kb): 2.2
Cross references: Insert lengths(kb): 2.200000047683716 Tissue: human Gene product: acetyl-CoA synthetase [CMV-His-ACS] Target Gene: acetyl-CoA synthetase |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/NotI--5.4,2.2; BamHI--7.6; HindIII--6.85,0.45,0.3; XhoI--7.6. Encodes a 731-amino acid fusion protein that contains the full length human ACS. Consists of an initiator methionine, six consecutive histidines, an anti-Xpress antibody epitope (DLYDDDDK), and aa 2-701 of human ACS. Expression is driven by the CMV promoter/enhancer in the vector. |
| References |
Luong A, et al. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins. J. Biol. Chem. 275: 26458-26466, 2000. PubMed: 10843999
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