| Vector Information |
Size (kb): 3.5000000000000000 Vector: pJ3omega (plasmid) Promoters: Promoter SV40 early Construction: pBR322, SV40 Marker(s):ampR Construct size (kb): 3.5 Features: marker(s): ampR
other: SV40 t IVS
promoter: SV40 early
replicon: SV40
replicon: pMB1
MCS: HindIII...BglII
terminator: SV40 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 2.35. A shuttle vector for expressing sequences cloned into the polylinker site under the control of the SV40 promoter. Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and SV40 (270-5171, early promoter, ori). |
| References |
Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225
Hartmut Land, personal communication
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