| 產品名稱 |
pZZ18 |
| 商品貨號 |
B203879 |
| Designations |
pZZ18 |
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
SJ Elledge |
| Applications |
mutation detection beta-galactosidase beta-D-galactosidase |
| Vector |
Construct size (kb): 12.39999961853027 |
| Insert |
DNA: genomic Insert lengths(kb): 4.599999904632568 Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) small subunit( ribonucleotide reductase, small subunit, ribonucleotide reductase M2 polypeptide) [CYC1] Target Gene: beta-galactosidase |
| Insert Size (kb) |
4.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--9.0, 3.9; EcoRI--6.0, 3.5, 3.3; SmaI--13.0. An RNR2-CYC1-lacZ transcriptional fusion construct wherein beta-galactosidase expression is regulated by the RNR2 promoter. Includes 0.6 kb upstream regulatory sequence of RNR2. Can be used to assay RNR2 activity, such as that induced by DNA damage. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Zhou Z, Elledge SJ. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131: 851-866, 1992. PubMed: 1516817
Zhou Z, Elledge SJ. DUN1 endoces a protein kinase that controls the DNA damage response in yeast. Cell 75: 1119-1127, 1993. PubMed: 8261511
|
