| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 2.5; PstI--5.3, 0.4, 0.2; EcoRI--2.8, 2.7, 0.4; HindIII--3.7, 1.0, 0.8, 0.3, 0.15; BamHI--4.9, 1.0, 0.18. The insert contains the following restriction sites (nt position according to the published sequence): PstI--159, 251, 630; EcoRI--300; PvuI--311; EcoRV--498; HindIII--754, 1077, 2030, 2280; BamHI--833, 1060; BglII--1828; SalI--2091; XbaI--2480. The total coding sequence is in an approximately 2.5 kb XbaI fragment. The sequence has been updated and corrected. |
| References |
McCormack T, et al. Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals. Proc. Natl. Acad. Sci. USA 87: 5227-5231, 1990. PubMed: 2367536
McCormack K, et al. Shaker K+ channel subunits form heteromultimeric channels with novel functional properties. Biochem. Biophys. Res. Commun. 171: 1361-1371, 1990. PubMed: 1699527
McCormack T, et al. Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals. Proc. Natl. Acad. Sci. USA 88: 4060, 1991. PubMed: 2023956
B Rudy, personal communication
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